microbial diseases laboratory branch
Test Name
Fungal Identification: Yeast
| Test Methodology | Identification of yeast species by MALDI-TOF mass spectrometry. |
|
Reflex Testing Criteria |
Reflex to fungal ITS sequencing if species ID is not resolved by MALDI-TOF. Send out to CDC for isolates that are not resolved by ITS sequencing. |
| Pre-Approval Required |
No |
|
Supplemental
Information and Required Form(s) |
Barcode submittal form 448 “Fungus Culture for Identification” or instructions for using the MDL Lab Web Portal (LWP) can be found at:
MDL Submission Instructions and Forms.
Instructions on completing the form at
MDL Form 448 |
|
Required Specimen Type(s) |
Pure culture on solid media from human source only. |
| Minimum Volume Required | Not Applicable |
|
Specimen Stability |
Room Temperature: acceptable Refrigerated: acceptable Frozen: DO NOT freeze |
|
Rejection Criteria |
|
|
Storage/Transport Conditions |
Grow isolate under appropriate conditions and verify purity prior to shipping.
Isolates may be stored under refrigeration or at ambient temperature prior to shipping. |
|
Transport Medium,
if applicable |
Avoid submitting fungal isolates on plates. Solid media in tube or flask with tightened screw cap and taped is preferable for submission. |
| Specimen Labeling | Two identifiers, including patient full name, and at least one other identifier (specimen accession number, date of birth, medical record number). |
|
Shipping Instructions and Specimen Handling Requirements
|
Ship cultures at ambient temperature (do not freeze). Follow the appropriate DOT/IATA approved shipping procedures. All the suspected
Coccidioides cultures should be shipped as a Category A Infectious Substance, Affecting Humans (UN2814). Ensure that culture isolates are sent in media flask or tubes containing a screw-top cap that is additionally sealed with parafilm or tape for additional protection against leaking.
Ship to: California Department of Public HealthMicrobial Diseases Laboratory ATTN: Mycology Unit C/O Specimen Receiving Room B106 850 Marina Bay Parkway, Richmond, CA 94804 |
|
Specimen Collection Instructions, if applicable |
Not Applicable |
| Turnaround Time | 4 days |
| Interferences & Limitations | Not Applicable |
| Reference Range | Not Applicable |
| Additional Information | Please contact the MDL Mycobacterial, Mycotic, and Parasitic Diseases Section for special consideration if any of the above criteria cannot be met. |
| MDL Point of Contact | For questions regarding submissions, please email
CDPHMDLMMP@cdph.ca.gov or call the MMPDS Section at (510) 412-3926 |